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Lee Biosolutions human blood purified pcrp

The effects of monomeric C‐reactive <t>proteins</t> <t>(mCRP)</t> on the amyloid beta (Aβ)42 secretion in primary cortical neurons. Postnatal day 0 to 2 cortical neurons from mice expressing different apolipoprotein E ( APOE ) genotype were isolated and cultured for 14 days. The neurons were treated with medium only or adding different concentrations of mCRP or pentameric CRP <t>(pCRP)</t> for 16 hours. A, Primary neurons from different APOE mice were treated with control medium versus different concentrations of mCRP. Cell culture media were collected and the expression levels of Aβ42 were detected by enzyme‐linkedimmunosorbent assay (ELISA). Values were the mean ± standard error (SE) from all experiments by using ANOVA with with Tukey's post hoc testing. Within each genotype, compared to the untreated neurons, differences of Aβ42 level with each concentration of mCRP are shown with statistical significance ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001, and ∗∗∗∗ P < .0001. Within each concentration of mCRP, the comparisons of different APOE genotypes were conducted by using analysis of variance (ANOVA) with Tukey's post hoc testing. Statistical significance is shown with # P < .05, ## P < .01, ### P < .001, and #### P < .0001. B, Primary neurons from different APOE mice were treated with control medium versus different concentrations of pCRP. Cell culture media were collected and the expression levels of Aβ42 were detected by ELISA. Values were the mean ± SE from all experiments by using ANOVA with with Tukey's post hoc testing. C, Real‐time polymerase chain reaction (PCR) assays were conducted to reveal the expressions of amyloid precursor protein (APP) in neurons. Values are expressed relative to untreated condition in each genotype, which were set as 1. Values were the mean ± SE from all experiments. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗ P < .05 and ∗∗ P < .01. D, Real‐time PCR assays were conducted to reveal the expressions of β‐secretase (BACE1) in neurons. Values are expressed relative to untreated condition in each genotype, which were set as 1. Values were the mean ± SE from all experiments. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗ P < .05. E, Western blot assays for the expressions of APP and BACE1 in different primary APOE neurons treated with mCRP were performed and quantified after normalization against β‐actin. The representative photographs are shown. Values were the mean ± SE. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗∗∗ P < .001

Human Blood Purified Pcrp, supplied by Lee Biosolutions, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+pleural+fluid/C-Reactive+Protein+(CRP)%2C+Human+Pleural+Fluid/pmc09270638-31-10-14
Average 93 stars, based on 5 article reviews

human blood purified pcrp - by Bioz Stars, 2026-09

93/100 stars

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1) Product Images from "Monomeric C‐reactive protein induces the cellular pathology of Alzheimer's disease"

Article Title: Monomeric C‐reactive protein induces the cellular pathology of Alzheimer's disease

Journal: Alzheimer's & Dementia : Translational Research & Clinical Interventions

doi: 10.1002/trc2.12319

The effects of monomeric C‐reactive proteins (mCRP) on the amyloid beta (Aβ)42 secretion in primary cortical neurons. Postnatal day 0 to 2 cortical neurons from mice expressing different apolipoprotein E ( APOE ) genotype were isolated and cultured for 14 days. The neurons were treated with medium only or adding different concentrations of mCRP or pentameric CRP (pCRP) for 16 hours. A, Primary neurons from different APOE mice were treated with control medium versus different concentrations of mCRP. Cell culture media were collected and the expression levels of Aβ42 were detected by enzyme‐linkedimmunosorbent assay (ELISA). Values were the mean ± standard error (SE) from all experiments by using ANOVA with with Tukey's post hoc testing. Within each genotype, compared to the untreated neurons, differences of Aβ42 level with each concentration of mCRP are shown with statistical significance ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001, and ∗∗∗∗ P < .0001. Within each concentration of mCRP, the comparisons of different APOE genotypes were conducted by using analysis of variance (ANOVA) with Tukey's post hoc testing. Statistical significance is shown with # P < .05, ## P < .01, ### P < .001, and #### P < .0001. B, Primary neurons from different APOE mice were treated with control medium versus different concentrations of pCRP. Cell culture media were collected and the expression levels of Aβ42 were detected by ELISA. Values were the mean ± SE from all experiments by using ANOVA with with Tukey's post hoc testing. C, Real‐time polymerase chain reaction (PCR) assays were conducted to reveal the expressions of amyloid precursor protein (APP) in neurons. Values are expressed relative to untreated condition in each genotype, which were set as 1. Values were the mean ± SE from all experiments. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗ P < .05 and ∗∗ P < .01. D, Real‐time PCR assays were conducted to reveal the expressions of β‐secretase (BACE1) in neurons. Values are expressed relative to untreated condition in each genotype, which were set as 1. Values were the mean ± SE from all experiments. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗ P < .05. E, Western blot assays for the expressions of APP and BACE1 in different primary APOE neurons treated with mCRP were performed and quantified after normalization against β‐actin. The representative photographs are shown. Values were the mean ± SE. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗∗∗ P < .001

Figure Legend Snippet: The effects of monomeric C‐reactive proteins (mCRP) on the amyloid beta (Aβ)42 secretion in primary cortical neurons. Postnatal day 0 to 2 cortical neurons from mice expressing different apolipoprotein E ( APOE ) genotype were isolated and cultured for 14 days. The neurons were treated with medium only or adding different concentrations of mCRP or pentameric CRP (pCRP) for 16 hours. A, Primary neurons from different APOE mice were treated with control medium versus different concentrations of mCRP. Cell culture media were collected and the expression levels of Aβ42 were detected by enzyme‐linkedimmunosorbent assay (ELISA). Values were the mean ± standard error (SE) from all experiments by using ANOVA with with Tukey's post hoc testing. Within each genotype, compared to the untreated neurons, differences of Aβ42 level with each concentration of mCRP are shown with statistical significance ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001, and ∗∗∗∗ P < .0001. Within each concentration of mCRP, the comparisons of different APOE genotypes were conducted by using analysis of variance (ANOVA) with Tukey's post hoc testing. Statistical significance is shown with # P < .05, ## P < .01, ### P < .001, and #### P < .0001. B, Primary neurons from different APOE mice were treated with control medium versus different concentrations of pCRP. Cell culture media were collected and the expression levels of Aβ42 were detected by ELISA. Values were the mean ± SE from all experiments by using ANOVA with with Tukey's post hoc testing. C, Real‐time polymerase chain reaction (PCR) assays were conducted to reveal the expressions of amyloid precursor protein (APP) in neurons. Values are expressed relative to untreated condition in each genotype, which were set as 1. Values were the mean ± SE from all experiments. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗ P < .05 and ∗∗ P < .01. D, Real‐time PCR assays were conducted to reveal the expressions of β‐secretase (BACE1) in neurons. Values are expressed relative to untreated condition in each genotype, which were set as 1. Values were the mean ± SE from all experiments. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗ P < .05. E, Western blot assays for the expressions of APP and BACE1 in different primary APOE neurons treated with mCRP were performed and quantified after normalization against β‐actin. The representative photographs are shown. Values were the mean ± SE. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗∗∗ P < .001

Techniques Used: Expressing, Isolation, Cell Culture, Control, Enzyme-linked Immunosorbent Assay, Concentration Assay, Real-time Polymerase Chain Reaction, Western Blot

The effects of monomeric C‐reactive proteins (mCRP) and pentameric CRP (pCRP) on the phosphorylated tau (p‐tau) expression in primary cortical neurons. Postnatal day 0 to 2 cortical neurons from mice expressing different apolipoprotein E ( APOE ) genotype were isolated and cultured for 14 days. The neurons were treated with medium only or adding different concentrations of mCRP for different time. A, Neurons from wild‐type (WT) mice or mice expressing different APOE genotypes were treated with control medium versus different concentrations of mCRP. The neurons were fixed, incubated with the p‐tau antibody, PHF1, to detect cellular tauopathy. The representative images were shown (scale bar: 50 μm). B, The level of tau phosphorylation was normalized by NeuN and quantitated. Values were the mean ± standard error (SE) from all experiments and compared using analysis of variance (ANOVA) with Tukey's post hoc testing. Within each genotype, differences with different concentrations of mCRP for each genotype were shown with statistical significance ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. Within the same concentration of mCRP treated neurons, the differences among different APOE neurons were compared with ANOVA with Tukey's post hoc testing. Statistical significance is shown # P < .05. C, The neurons were treated with control medium or 5 μg/mL mCRP for different incubation time. The level of tau phosphorylation was normalized by NeuN and quantitated. Values were the mean ± SE from all experiments by using ANOVA with Tukey's post hoc testing. Within each genotype, differences with statistical significance for each genotype were shown with ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. D, The neurons were treated with control medium versus different concentrations of pCRP. The level of tau phosphorylation was normalized by NeuN and quantitated. Values were the mean ± SE from all experiments. E, The neurons were treated with control medium versus 5 μg/mL pCRP for different incubation time. The level of tau phosphorylation was normalized by NeuN and quantitated. Values were the mean ± SE from all experiments. F, Real‐time polymerase chain reaction (PCR) assay was conducted to reveal the expressions of CDK5 in neurons. Values are expressed relative to untreated APOE ε2 mouse primary neurons, which were set as 1. Values were the mean ± SE from all experiments by using ANOVA with Tukey's post hoc testing. Within each genotype, differences with statistical significance were shown with ∗ P < .05. G, Real‐time PCR assay was conducted to reveal the expressions of GSK3β in neurons. Values are expressed relative to untreated APOE ε2 mouse primary neurons, which were set as 1. Values were the mean ± SE from all experiments by using ANOVA with Tukey's post hoc testing. Within each genotype, differences with statistical significance were shown with ∗ P < .05 and ∗∗ P < .01. H, Western blot assays for the expressions of GSK3β, pGSK3β, and CDK5 in neurons were performed and quantified after normalization against β‐actin. The representative photographs are shown. Values were the mean ± SE. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗ P < .05 and ∗∗ P < .01

Figure Legend Snippet: The effects of monomeric C‐reactive proteins (mCRP) and pentameric CRP (pCRP) on the phosphorylated tau (p‐tau) expression in primary cortical neurons. Postnatal day 0 to 2 cortical neurons from mice expressing different apolipoprotein E ( APOE ) genotype were isolated and cultured for 14 days. The neurons were treated with medium only or adding different concentrations of mCRP for different time. A, Neurons from wild‐type (WT) mice or mice expressing different APOE genotypes were treated with control medium versus different concentrations of mCRP. The neurons were fixed, incubated with the p‐tau antibody, PHF1, to detect cellular tauopathy. The representative images were shown (scale bar: 50 μm). B, The level of tau phosphorylation was normalized by NeuN and quantitated. Values were the mean ± standard error (SE) from all experiments and compared using analysis of variance (ANOVA) with Tukey's post hoc testing. Within each genotype, differences with different concentrations of mCRP for each genotype were shown with statistical significance ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. Within the same concentration of mCRP treated neurons, the differences among different APOE neurons were compared with ANOVA with Tukey's post hoc testing. Statistical significance is shown # P < .05. C, The neurons were treated with control medium or 5 μg/mL mCRP for different incubation time. The level of tau phosphorylation was normalized by NeuN and quantitated. Values were the mean ± SE from all experiments by using ANOVA with Tukey's post hoc testing. Within each genotype, differences with statistical significance for each genotype were shown with ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. D, The neurons were treated with control medium versus different concentrations of pCRP. The level of tau phosphorylation was normalized by NeuN and quantitated. Values were the mean ± SE from all experiments. E, The neurons were treated with control medium versus 5 μg/mL pCRP for different incubation time. The level of tau phosphorylation was normalized by NeuN and quantitated. Values were the mean ± SE from all experiments. F, Real‐time polymerase chain reaction (PCR) assay was conducted to reveal the expressions of CDK5 in neurons. Values are expressed relative to untreated APOE ε2 mouse primary neurons, which were set as 1. Values were the mean ± SE from all experiments by using ANOVA with Tukey's post hoc testing. Within each genotype, differences with statistical significance were shown with ∗ P < .05. G, Real‐time PCR assay was conducted to reveal the expressions of GSK3β in neurons. Values are expressed relative to untreated APOE ε2 mouse primary neurons, which were set as 1. Values were the mean ± SE from all experiments by using ANOVA with Tukey's post hoc testing. Within each genotype, differences with statistical significance were shown with ∗ P < .05 and ∗∗ P < .01. H, Western blot assays for the expressions of GSK3β, pGSK3β, and CDK5 in neurons were performed and quantified after normalization against β‐actin. The representative photographs are shown. Values were the mean ± SE. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗ P < .05 and ∗∗ P < .01

Techniques Used: Expressing, Isolation, Cell Culture, Control, Incubation, Phospho-proteomics, Concentration Assay, Real-time Polymerase Chain Reaction, Western Blot

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Article Title: Fluorescent ozone sensor
Article Snippet: Human pleural fluid was purchased from Lee Biosolutions and used as received.

Article Title: Specific fluorogenic probes for ozone in biological and atmospheric samples
Article Snippet: Human pleural fluid was purchased from Lee Biosolutions and used as received.

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Journal: Alzheimer's & Dementia : Translational Research & Clinical Interventions

Article Title: Monomeric C‐reactive protein induces the cellular pathology of Alzheimer's disease

doi: 10.1002/trc2.12319

Figure Lengend Snippet: The effects of monomeric C‐reactive proteins (mCRP) on the amyloid beta (Aβ)42 secretion in primary cortical neurons. Postnatal day 0 to 2 cortical neurons from mice expressing different apolipoprotein E ( APOE ) genotype were isolated and cultured for 14 days. The neurons were treated with medium only or adding different concentrations of mCRP or pentameric CRP (pCRP) for 16 hours. A, Primary neurons from different APOE mice were treated with control medium versus different concentrations of mCRP. Cell culture media were collected and the expression levels of Aβ42 were detected by enzyme‐linkedimmunosorbent assay (ELISA). Values were the mean ± standard error (SE) from all experiments by using ANOVA with with Tukey's post hoc testing. Within each genotype, compared to the untreated neurons, differences of Aβ42 level with each concentration of mCRP are shown with statistical significance ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001, and ∗∗∗∗ P < .0001. Within each concentration of mCRP, the comparisons of different APOE genotypes were conducted by using analysis of variance (ANOVA) with Tukey's post hoc testing. Statistical significance is shown with # P < .05, ## P < .01, ### P < .001, and #### P < .0001. B, Primary neurons from different APOE mice were treated with control medium versus different concentrations of pCRP. Cell culture media were collected and the expression levels of Aβ42 were detected by ELISA. Values were the mean ± SE from all experiments by using ANOVA with with Tukey's post hoc testing. C, Real‐time polymerase chain reaction (PCR) assays were conducted to reveal the expressions of amyloid precursor protein (APP) in neurons. Values are expressed relative to untreated condition in each genotype, which were set as 1. Values were the mean ± SE from all experiments. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗ P < .05 and ∗∗ P < .01. D, Real‐time PCR assays were conducted to reveal the expressions of β‐secretase (BACE1) in neurons. Values are expressed relative to untreated condition in each genotype, which were set as 1. Values were the mean ± SE from all experiments. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗ P < .05. E, Western blot assays for the expressions of APP and BACE1 in different primary APOE neurons treated with mCRP were performed and quantified after normalization against β‐actin. The representative photographs are shown. Values were the mean ± SE. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗∗∗ P < .001

Article Snippet: On day 14 of culture, E. coli recombinant mCRP or human blood purified pCRP (LeeBio, #140‐11A) was added in cell culture medium followed by cell incubation.

Techniques: Expressing, Isolation, Cell Culture, Control, Enzyme-linked Immunosorbent Assay, Concentration Assay, Real-time Polymerase Chain Reaction, Western Blot

Journal: Alzheimer's & Dementia : Translational Research & Clinical Interventions

Article Title: Monomeric C‐reactive protein induces the cellular pathology of Alzheimer's disease

doi: 10.1002/trc2.12319

Figure Lengend Snippet: The effects of monomeric C‐reactive proteins (mCRP) and pentameric CRP (pCRP) on the phosphorylated tau (p‐tau) expression in primary cortical neurons. Postnatal day 0 to 2 cortical neurons from mice expressing different apolipoprotein E ( APOE ) genotype were isolated and cultured for 14 days. The neurons were treated with medium only or adding different concentrations of mCRP for different time. A, Neurons from wild‐type (WT) mice or mice expressing different APOE genotypes were treated with control medium versus different concentrations of mCRP. The neurons were fixed, incubated with the p‐tau antibody, PHF1, to detect cellular tauopathy. The representative images were shown (scale bar: 50 μm). B, The level of tau phosphorylation was normalized by NeuN and quantitated. Values were the mean ± standard error (SE) from all experiments and compared using analysis of variance (ANOVA) with Tukey's post hoc testing. Within each genotype, differences with different concentrations of mCRP for each genotype were shown with statistical significance ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. Within the same concentration of mCRP treated neurons, the differences among different APOE neurons were compared with ANOVA with Tukey's post hoc testing. Statistical significance is shown # P < .05. C, The neurons were treated with control medium or 5 μg/mL mCRP for different incubation time. The level of tau phosphorylation was normalized by NeuN and quantitated. Values were the mean ± SE from all experiments by using ANOVA with Tukey's post hoc testing. Within each genotype, differences with statistical significance for each genotype were shown with ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. D, The neurons were treated with control medium versus different concentrations of pCRP. The level of tau phosphorylation was normalized by NeuN and quantitated. Values were the mean ± SE from all experiments. E, The neurons were treated with control medium versus 5 μg/mL pCRP for different incubation time. The level of tau phosphorylation was normalized by NeuN and quantitated. Values were the mean ± SE from all experiments. F, Real‐time polymerase chain reaction (PCR) assay was conducted to reveal the expressions of CDK5 in neurons. Values are expressed relative to untreated APOE ε2 mouse primary neurons, which were set as 1. Values were the mean ± SE from all experiments by using ANOVA with Tukey's post hoc testing. Within each genotype, differences with statistical significance were shown with ∗ P < .05. G, Real‐time PCR assay was conducted to reveal the expressions of GSK3β in neurons. Values are expressed relative to untreated APOE ε2 mouse primary neurons, which were set as 1. Values were the mean ± SE from all experiments by using ANOVA with Tukey's post hoc testing. Within each genotype, differences with statistical significance were shown with ∗ P < .05 and ∗∗ P < .01. H, Western blot assays for the expressions of GSK3β, pGSK3β, and CDK5 in neurons were performed and quantified after normalization against β‐actin. The representative photographs are shown. Values were the mean ± SE. Within each genotype, compared to the untreated neurons, differences with statistical significance were shown with ∗ P < .05 and ∗∗ P < .01

Article Snippet: On day 14 of culture, E. coli recombinant mCRP or human blood purified pCRP (LeeBio, #140‐11A) was added in cell culture medium followed by cell incubation.

Techniques: Expressing, Isolation, Cell Culture, Control, Incubation, Phospho-proteomics, Concentration Assay, Real-time Polymerase Chain Reaction, Western Blot

Correlation between mCRP and pCRP concentrations. The correlation between mCRP and pCRP concentration was calculated. Although high-pCRP samples tended to exhibit high mCRP levels, no positive correlation was observed ( R 2 = 0.08).

Journal: Frontiers in Immunology

Article Title: mCRP as a Biomarker of Adult-Onset Still’s Disease: Quantification of mCRP by ELISA

doi: 10.3389/fimmu.2022.938173

Figure Lengend Snippet: Correlation between mCRP and pCRP concentrations. The correlation between mCRP and pCRP concentration was calculated. Although high-pCRP samples tended to exhibit high mCRP levels, no positive correlation was observed ( R 2 = 0.08).

Article Snippet: Briefly, mCRP were generated from commercially available human pCRP (human pleural fluid, Lee BioSolutions, Maryland Heights, MO, USA) as described previously ( ).

Techniques: Concentration Assay

Plasma mCRP concentration. Plasma mCRP concentration was measured in patients with AOSD (n = 20), PMR (n = 20), RA (n = 30), infection (n = 50), and in control subjects (n = 30) using the developed ELISA. (A) Plasma mCRP concentration in each group. (B) mCRP (×1,000)/pCRP ratio. Statistical analysis was performed using a Kruskal-Wallis test followed by Dunn’s post-test comparison. Data are shown as means +/- SD. * P < 0.05.

Journal: Frontiers in Immunology

Article Title: mCRP as a Biomarker of Adult-Onset Still’s Disease: Quantification of mCRP by ELISA

doi: 10.3389/fimmu.2022.938173

Figure Lengend Snippet: Plasma mCRP concentration. Plasma mCRP concentration was measured in patients with AOSD (n = 20), PMR (n = 20), RA (n = 30), infection (n = 50), and in control subjects (n = 30) using the developed ELISA. (A) Plasma mCRP concentration in each group. (B) mCRP (×1,000)/pCRP ratio. Statistical analysis was performed using a Kruskal-Wallis test followed by Dunn’s post-test comparison. Data are shown as means +/- SD. * P < 0.05.

Article Snippet: Briefly, mCRP were generated from commercially available human pCRP (human pleural fluid, Lee BioSolutions, Maryland Heights, MO, USA) as described previously ( ).

Techniques: Concentration Assay, Infection, Enzyme-linked Immunosorbent Assay

Time course of changes in plasma mCRP level in AOSD patients. Dynamic changes in plasma mCRP concentration in AOSD patients after immunosuppressive therapy were evaluated (n = 9) at the indicated time points. Plasma mCRP levels decreased more rapidly than pCRP levels following immunosuppressive therapy. Statistical analysis was performed using a Wilcoxon signed-rank test. * P < 0.05. mCRP concentration on day 0 and indicated days were compared.

Journal: Frontiers in Immunology

Article Title: mCRP as a Biomarker of Adult-Onset Still’s Disease: Quantification of mCRP by ELISA

doi: 10.3389/fimmu.2022.938173

Figure Lengend Snippet: Time course of changes in plasma mCRP level in AOSD patients. Dynamic changes in plasma mCRP concentration in AOSD patients after immunosuppressive therapy were evaluated (n = 9) at the indicated time points. Plasma mCRP levels decreased more rapidly than pCRP levels following immunosuppressive therapy. Statistical analysis was performed using a Wilcoxon signed-rank test. * P < 0.05. mCRP concentration on day 0 and indicated days were compared.

Article Snippet: Briefly, mCRP were generated from commercially available human pCRP (human pleural fluid, Lee BioSolutions, Maryland Heights, MO, USA) as described previously ( ).

Techniques: Concentration Assay

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